Model NO.: ALP
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*40 mL, R2: 1*20 mL
Origin: Jinan, Shandong
Model NO.: ALP
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*40 mL, R2: 1*20 mL
Origin: Jinan, Shandong
The alkaline phosphatase (ALP) assay kit is intended for the accurate and direct quantitative measurement of ALP activity in human plasma. ALP measurement is useful in the diagnosis of liver, bile diseases and bone disorders.
Test principle
4-Nitrophenyl Phosphate (4-NPP) can be dephosphorylated by ALP and converted into 4- Nitrophenol (4-NP), which turns yellow due to it quinoid structure formed in alkaline environment. The unit activity of ALP can be calculated from the increasing rate of absorbance detected at a wavelength of 405 nm.
Product Features
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1. Linear range of ALP activity: [3, 500] U/L
Linear correlation coefficient ∣r∣≥ 0.990Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≤ 0.80
3. Changing rate of absorbance of blank ΔA/min ≤ 0.05 Â
4. Sensitivity
Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific ALP sample of activity of 223 U/L.5. Accuracy
Relative deviation is within ±10%.6. Precision
Intra CV% ≤ 6.0%; inter CV% ≤8.0%.Technical Parameters
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| Temperature | 37ºC | Optical path length (OPL) | 1.0 cm |
| Main wavelength | 405 nm | Secondary wavelength | 480 nm (Optional) |
| Method | Velocity method | Reaction direction | Positive |
| Sample | 4 μL | R1 | 200 μL |
| R2 | 50 μL | Delay time | 50 s |
| Reaction time | 180 s | Â | Â |
Packing Specifications
R1: 2×40 mL, R2: 1×20 mL
R1: 4×60 mL, R2: 4×15 mL
R1: 1×40 mL, R2: 1×10 mL (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
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The alkaline phosphatase (ALP) assay kit is intended for the accurate and direct quantitative measurement of ALP activity in human plasma. ALP measurement is useful in the diagnosis of liver, bile diseases and bone disorders.
Test principle
4-Nitrophenyl Phosphate (4-NPP) can be dephosphorylated by ALP and converted into 4- Nitrophenol (4-NP), which turns yellow due to it quinoid structure formed in alkaline environment. The unit activity of ALP can be calculated from the increasing rate of absorbance detected at a wavelength of 405 nm.
Product Features
Â
1. Linear range of ALP activity: [3, 500] U/L
Linear correlation coefficient ∣r∣≥ 0.990Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≤ 0.80
3. Changing rate of absorbance of blank ΔA/min ≤ 0.05 Â
4. Sensitivity
Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific ALP sample of activity of 223 U/L.5. Accuracy
Relative deviation is within ±10%.6. Precision
Intra CV% ≤ 6.0%; inter CV% ≤8.0%.Technical Parameters
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| Temperature | 37ºC | Optical path length (OPL) | 1.0 cm |
| Main wavelength | 405 nm | Secondary wavelength | 480 nm (Optional) |
| Method | Velocity method | Reaction direction | Positive |
| Sample | 4 μL | R1 | 200 μL |
| R2 | 50 μL | Delay time | 50 s |
| Reaction time | 180 s | Â | Â |
Packing Specifications
R1: 2×40 mL, R2: 1×20 mL
R1: 4×60 mL, R2: 4×15 mL
R1: 1×40 mL, R2: 1×10 mL (Sample)
Storage and stability
Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 15 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
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