How is the dialysis bag activated? (methodology)

The common type of dialysis bag mainly refers to the dialysis bag produced by the United States Carbonation Company (Viskase). The dry type and the dialysis bag have high levels of heavy metal ions and sulfides. It is possible to remove some of the parts without proper methods. This article mainly introduces General dialysis bag handling and use methods. How is the dialysis bag activated? (method)

A , technical parameters:
1.PH stable range: 5-9
2. Contaminant levels: sulfide <0.3%; heavy metal <50ppm
3. Chemical compatibility: Compatible with many salts, such as CaCl2, (NH4)2SO4; also compatible with water solvents and organic solvents commonly used in molecular biology and enzymology, such as isopropanol, ethanol and acetone.
4. Temperature resistance: can be boiled and autoclavable.
5. Protein adsorption: The amount of protein adsorbed per gram of dialysis bag is less than 1ng

B , storage conditions:
The dialysis bag before untreated can be stored at 0-43 degrees, sealed and packaged to prevent cracking; the disposable dialysis bag needs to be placed in the D-storage solution and placed at 4-37 degrees. .

C , pre-treatment method of dialysis bag:

1. Cut the dialysis bag into small pieces of appropriate length (10-20 cm);

2. Boil the dialysis bag in a large volume (500 mL) of 2% (w/v) sodium bicarbonate and 1 mmol/L EDTA.2Na (pH=8.0) for 10 min, rinse thoroughly with distilled water and then place in 500 mL of 1 Boil for 10 min in mmol/L EDTA.2Na (pH=8.0);

3. Remove the dialysis bag thoroughly with distilled water.

4. If not used, the dialysis bag should be completely immersed in the dialysis bag storage solution and stored at 4-37 degrees.

5. Before dialysis bag is soaked in the dialysis bag storage solution, the dialysis bag must be filled with water and then drained, cleaned, and rinsed three times inside and outside.

The experimental requirements are not high, you can use the simple treatment method: you can use it in boiling water for 10 minutes.

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