Environmental protection edible mushroom strain preservation method

The preservation methods of edible mushroom strains generally include: refrigerator preservation, paraffin sealing, and sand burying. They have their own characteristics, but they all control their growth, reduce respiration and metabolism by changing the environmental conditions such as temperature and carbon dioxide concentration in the air. To achieve the purpose of preservation. These methods have great differences in the length of time for preservation, the degree of contamination, and the requirements for the environment. Some of the operating techniques are relatively complex and difficult for general personnel to grasp. We trained on Pleurotus ostreatus 1015 strain at the same time, it was salt-tolerant training, is the ordinary PDA culture medium salinization, formulated into a concentration of 1.0% to 1.5% of the solution, autoclave 45 to 60 minutes Cooling is made into an incline, inoculated under aseptic conditions, sealed with a tampon, and incubated in a constant temperature chamber. After the culture is mature, each group of 10 pieces is put into an ethylene bag and preserved at room temperature for 9 months. The survival rate is more than 95%, which is almost the same as other preservation. After the tube was inoculated, the survival rate of the inoculation block was high. The main reason is that the salt solution has a high permeability and has a strong inhibition and killing effect on the germination of the spores, thereby reducing or even avoiding contamination during the preservation, and the sodium ion is the process of the mycelium germination of edible fungi. Indispensable, at the same time, salt also contains magnesium, calcium and other ions necessary for the normal metabolism of edible fungi, also has a certain role in promoting the growth of mycelium, and in the tube, rejuvenation process, due to the existence of poor water potential, inoculum block Strong water absorption ability, easy recovery, high survival rate, but it should be noted that in the inoculation, the second and third grades, until the cultivation process, it is necessary to gradually reduce the salt content of the culture material in order to form a water potential difference, promote mycelial germination, reduce pollution, Improve the yield. This method is easy to learn and is very suitable for small and medium-sized mushroom farmers. Interested friends may wish to try.

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