Q. What are the components in the Platinum ELISA Kit?
What do Platinum ELISA Kits contain?
A , The kit contains all of the components used in the ELISA assay and contains an optimized pre-coated ELISA plate. All reagents necessary to perform 1 or 10 plate ELISA assays, including optimized capture-antibody pre-coated plates.
Q. Which sample types are used for the Platinum ELISA Kit?
Which samples are suitable for Platinum ELISA Kits?
A , Most Platinum ELISA kits can detect common ELISA samples such as serum, plasma, cell supernatant and other body fluid samples. For details, you can view the instructions for the corresponding product.
Most ELISAs are suitable for serum, plasma, cell culture supernatants and other body fluids. Detailed information is given in the product manual for each individual kit.
Q. Is it necessary to make a double hole in the calibration and sample using the Platinum ELISA kit?
Do I have to run the Platinum ELISA standards and samples in duplicates?
A , Not a must. However, for accuracy of results, we strongly recommend that you repeat the standard curve and sample. No, but it is highly recommended to make at least double determinations of each sample and standard point.
Q. Can the components in different kits or different batches of Platinum Kit be mixed?
Is it possible to use the reagents of other Platinum ELISA kits/lots?
A , No.
The reagents within the Platinum ELISA kits are not interchangeable between different lots of the same kit or between kits against different analytes.
Q. Can the different batch kit instructions be used for reference?
Can I always use the same manual for different ELISA kit lot numbers?
A, Â Â Not recommended. It is recommended that you refer to the product manual received with the goods.
We recommend that you use the manual included with the Platinum ELISA kit.
Q. Is it necessary to wash the ELISA plate before using the Platinum ELISA kit?
Is it necessary to wash the Platinum ELISA plates before usage?
A, Â Â Â Â Initial washing is not required, but for more accurate results, we recommend that you perform this step.
An initial washing step is not necessary but recommended as accuracy may be increased.
Q. Can the incubation time in the ELISA kit instructions be extended or shortened at will?
Are shorter or longer incubation times possible?
A , In order to obtain more accurate results, it is highly recommended that you follow the recommended incubation time in the product manual. Incubation times should be followed exactly as stated in the product manual to ensure optimal Platinum ELISA test performance.
Q. When using Platinum ELISA to test samples, can I directly test without diluting my own samples? Can I omit the dilution of my Platinum ELISA samples with assay buffer/sample diluent when performing the assay?
A , It is recommended to follow the instructions for dilution.
We recommend following the instructions for sample dilutions as provided in the manual. We cannot guarantee optimal performance of the assay if internal assay dilutions as instructed were omitted.
Q. What should I do if I find that the components in the kit are not enough when tested? What if it does not look like the supplied reagent volume is sufficient for running the Platinum ELISA?
A , If the reagent appears to be small, be sure to centrifuge briefly before use to ensure that the liquid in the tube is collected at the bottom of the tube. In case of small reagent volumes, please spin down vial before use to make sure to all contents are collected in the bottom of the tube.
Q. Can I save it in other ways besides the storage method required by the kit?
Is it possible to store the reagents other than indicated?
A , Not recommended. If the kit is not stored as described, the quality of the Platinum ELISA kit cannot be guaranteed . Performance of Platinum ELISA cannot be guaranteed if reagents are not stored as indicated.
Q. Is there a test value for a normal sample in the Platinum ELISA Kit? Do you have information regarding the normal values ​​detectable with your Platinum ELISA Kit?
A , You can find the normal sample expectation in the "Expected Values" section of the corresponding product manual . However, these values ​​are obtained after a limited sample of our laboratory tests. We strongly recommend that your lab obtain the normal values ​​of your samples by testing your research population. You can find expected "normal levels" listed in the Platinum ELISA Kit manual under "Expected Values". Please be aware that these normal values ​​were obtained in our laboratories with a limited number of samples. We strongly suggest that each laboratory perform studies in order To establish normal range for their sample populations.
The above information is from the official website of eBioscience :
Http://us.ebioscience.com/resources/faq/elisa-faq.htm#Platinum-ELISA-Kits .
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