Experiment: Glycyrrhiza radicans Chinese herbal medicine sample grinding
Experimental materials: Chinese herbal medicines such as licorice and puerarin
Experimental purpose: to measure the content of glycyrrhizic acid, followed by component analysis experiments
Experimental equipment: TL2020 high-throughput tissue grinder
Experimental steps:
1. Before the experiment, first smash the licorice or Pueraria into small pieces, or pieces, and prepare for automatic grinding;
2. Take no more than one third of the total volume of the grinding tank and place it in a stainless steel grinding tank;
2. Put stainless steel grinding beads and quickly place the grinding tank on the Dingyuan TL2020 high-flux tissue grinding machine;
3. Under the condition of constant rotation speed, it can be automatically ground for several minutes, intermittently stopped for several minutes, automatically ground again for several minutes, and intermittently stopped for several minutes to obtain sample powder.
昊诺斯 users:
This case is the scope of chemical analysis. After grinding, the content of glycyrrhizic acid can be determined in the liquid phase. I used to extract 0.2 g of licorice powder into a 100 ml volumetric flask with a one-to-one aqueous solution of methanol.
昊诺斯宾宾:
Teacher Shi is quite familiar. The follow-up experiment of this grinding sharing case is used for component analysis and dry grinding into powder.
昊诺斯 users:
Yep.
昊诺斯宾宾:
This user company is a Chinese medicine decoction product, which is usually measured before mass production.
昊诺斯 users:
This is still very convenient.
昊诺斯宾宾:
Generally, grind some licorice, puerarin, Dendrobium, etc., and history teacher can also share with us the samples you generally grind.
昊诺斯 users:
The standard tested was ammonium glycyrrhizinate.
昊诺斯宾宾:
Is it also necessary to grind?
昊诺斯 users:
No need to convert to glycyrrhizic acid. After dissolving 0.2 ml of the powder in 100 ml, it can be detected after passing through the filter.
昊诺斯宾宾:
You can use a standard solution or the like. You can use our tissue grinder later if you encounter a sample that requires pre-grinding. The teacher who grinds the Chinese herbal medicine piece told us that the fiber part of the sample was discarded altogether when the tissue grinder was not used. Now it is automatically ground with the Dingyuan TL2020 high-throughput tissue grinder, and the fiber samples can be broken especially well.
昊诺斯 users:
Yep.
昊诺斯宾宾:
Generally grinding such a slightly hard or tough sample, it is more suitable to use a 50ml stainless steel grinding jar. The grinding beads can be selected from steel balls, like the 25mm stainless steel grinding beads in this experimental case.
昊诺斯宾宾:
In fact, the pre-processing of the experiment is really very important. It affects the success and accuracy of the follow-up experiment all the time. Now there is an automated tissue grinder instead of the manual. The teachers who have used it all reflect the efficiency of the experiment. The second is to help the subsequent experiments. In today's sharing case, Chinese herbal medicines are ground by dry grinding and require intermittent grinding.
昊诺斯 users:
Heat dissipation is the key.
昊诺斯宾宾:
Yes, when grinding for more than 3 minutes, it can be stopped after a few minutes to avoid overheating.
昊诺斯 users:
The amount of sample in the tissue grinder should not be excessive.
昊诺斯宾宾:
Yes, generally no more than one-third of the total volume of the grinding tank.
昊诺斯 users:
We are planting, but because we are testing viruses, we need to extract RNA, leaves or bulbs and add liquid nitrogen to grind with a tissue grinder.
昊诺斯宾宾:
Then you are liquid nitrogen grinding, and RNA is generally required to assist with liquid nitrogen.
昊诺斯 users:
Correct.
昊诺斯宾宾:
The horizontal tissue grinder is ideal for liquid nitrogen assisted grinding. There is no need to worry about liquid nitrogen dripping into the motor, ensuring safety and machine life.
昊诺斯 users:
correct.
昊诺斯宾宾:
In the usual experiment, if you encounter a hard and hard-to-wear sample teacher, how do you usually grind it, can you simply share it with us? For example, it will use a few beads, the size of different sizes to grind, or for example, increase the speed to extend the grinding time and so on?
昊诺斯 users:
Increase the speed to extend the grinding time.
昊诺斯 users:
Does the sample need to be dried?
昊诺斯宾宾:
Yes, the dryness of the sample during the dry grinding process will be ground into powder faster.
昊诺斯 users:
We don't dry, we need fresh samples.
昊诺斯宾宾:
Well, wet grinding and liquid nitrogen grinding do not require special drying, but it is necessary to dry the viscous sample to avoid insufficient grinding when grinding. When grinding ore, it has been sticky after being ground into powder, and it took a little effort to take it out. For example, magnesium sulfate is also a dry grinding machine. The large 50ml grinding jar is used, and the effect is quite good.
昊诺斯宾宾:
Basically, the dry grinding in today's case is mostly used in Chinese herbal medicines (licorice, puerarin, python, dried snake meat, etc.), or grinding of ore, teeth, etc., subsequent experiments are mostly the determination of ingredients, teachers with such experiments Can refer to. There are a lot of teachers in the group who haven't seen our tissue grinder yet. We have a video of the operation guide. I will give you a chance to share it later.
Key words: TL2020 high-throughput tissue grinder, Ding Yiyuan
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