[Introduction] In recent years, the quality of food has been exposed, and there are also high sugar and high fat problems in baby milk food. Many people are "talking about fat". Infants eating excessive amounts of sugar and fat, in addition to causing obesity, can also affect the baby's calcium absorption, damage the baby's cardiovascular, leading to abnormal development. So, how to detect the fat content in infant milk food?
[ GBB ] In 2011, the state announced GB5413.3-2010 "Determination of fat in infant foods and dairy products", clearly indicating that two methods can be used to detect fat. The first is the Mohs method, which is applied to the determination of fat in pasteurized milk, sterilized milk, raw milk, fermented milk, prepared milk, milk powder, condensed milk, cream, cream, cheese and infant formula. The principle is alkaline hydrolysis; the second method is the Gabor method , which is suitable for the determination of fat in pasteurized milk, sterilized milk and raw milk, and the principle is acid hydrolysis.
The focus here is on the Gabriel method that can quickly detect fat in dairy products.
[Test method ]
Gabriel
[Detection principle]
The principle of gabacuric acid hydrolysis is to add sulfuric acid to the milk to destroy the latex and the outer membrane of the protein covering the fat globule, and measure the volume after centrifuging the fat.
[Experimental reagent material]
Experimental reagents:
1) Sulfuric acid (H 2 SO 4 ): analytically pure, having a density of about 1.84 g/L.
2) Isoamyl alcohol (C 5 H 12 O): analytical pure experimental instrument:
| equipment name | Brand | model |
| Cream centrifuge (with heating function, with cover tube adapter) | Swiss Gabriel Instruments Gerber Instruments | Gerber centrifuge Universal |
| Gabor creamer | Swiss Gabriel Instruments Gerber Instruments | Milk Butyrometer Original Gerber |
| Single-label breast tube | Swiss Gabriel Instruments Gerber Instruments | 03.1003 (10.75mL) |
| Water bath | Swiss Gabriel Instruments Gerber Instruments or Bibby Stuart | Waterbath Butytherm mini Or SWB24D |
Creamy centrifuge ç›–åšä¹³è„‚水水槽
[Analysis step]
1) Add 10 mL of sulfuric acid (11.1) to the Gabor creamer, then carefully and accurately add 10.75 mL of the sample along the tube wall, so that the sample is not mixed with sulfuric acid, then add 1 mL of isoamyl alcohol (11.2) and apply the rubber. Plug the bottle down and wrap it in a cloth to prevent it from rushing out.
2) Shake vigorously to make a uniform brown liquid, let stand for a few minutes (the bottle mouth is down), set it in a water bath at 65 °C ~ 70 °C for 5 min, remove it and place it in a cream centrifuge to centrifuge at 1100 rpm. Min, then placed in 65 ° C ~ 70 ° C water bath water for 5 min (note that the water bath surface should be higher than the fat layer of the fat meter).
3) Immediate reading, which is the percentage of fat.
* Swiss Gabriel Instruments is the founder of this well-known Gabriel . Since its introduction in 1892, Gabriel has been popular in the world for more than 120 years.
About Ubi and British Bibby / German ART / German CAT / Swiss Gerber Instruments
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Guangzhou Special Instrument Technology Co., Ltd. specializes in general instruments such as laboratory sample preparation such as agitator/dispersive emulsifier, analytical instruments such as melting point/photometer/freezing point instrument, and life science instruments such as PCR. As the first generation of British Bibi in South China, Guangdong, Guangxi, Sichuan, Chongqing, Yunnan, Hainan, Guizhou and Tibet are our services. The company is also the German ART, Germany CAT, the first generation of Swiss Gerber Instruments in China.
※British BIBBY was established in the 1950s as the largest laboratory scientific instrument manufacturer in the UK with four sub-brands: Stuart, Techne, Jenway, Electrothermal. Focus on general laboratory instruments such as sample preparation (eg melting point apparatus, stirrer, mixer, shaker, incubator, dry bath/nitrogen blower, water bath, colony counter, pure water still), molecular biology Research equipment (gene amplification instrument PCR, fluorescence quantification, hybridization box); spectrophotometer / ultra-micro UV and other analytical instruments, and parallel reaction workstation related products.
※ Germany ART was founded in the last century and is the most professional dispersing emulsifier in Germany and the world. Top-grade dispersing emulsified products range from laboratory instruments, pilot products to industrial equipment. There are hundreds of types of dispersing heads; the fields of application cover chemical, cosmetic, pharmaceutical, food, environmental protection and other fields.
※German CAT was founded in the 1950s and is one of the experts in German sample preparation instruments. It is known for its “quality stabilityâ€. Its top-mounted mixers range from hand-held, teaching, to scientific research, and high-viscosity. It is the representative product line of CAT.
※Gerber Instruments has a history of more than 120 and is a typical representative of the dairy food industry. Its products are freezing point, cream centrifuge, food-specific PH meter, outflow viscometer, etc., which are popular in Europe and other mainland countries.
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