Routine disinfection of pig farms should use a broad-spectrum, high-efficiency, non-toxic, non-irritating disinfectant; regular use of different types of disinfectants cannot use disinfectants of the same nature for a long time. The pig farm's living area, office area and feed processing area should be kept clean and disinfected once a week. A disinfection tank shall be set at the gate of the farm, and 2%~3% of fire alkali water shall be placed in the pool, and it shall be replaced once a week. For the disinfection of personnel, the entry staff must enter the production area after changing the overalls, shoes and caps in the locker room. The pig house was sterilized. After the herd was transferred out, the pig house was completely sterilized, and the sewers, sewage drains, walls, floors, and ditch were completely disinfected. Pig body disinfection, disinfection of the pig must use non-toxic, non-irritating and non-corrosive disinfectants, and the sheds and breeding houses are sterilized twice a week. Disinfection of utensils, sweeping utensils and feeding utensils in pig houses should be stored separately, and should be cleaned and disinfected after each use; commonly used therapeutic instruments such as syringe needles should be boiled and disinfected after each use, and at the same time, they should be treated as a pig. A needle; sterilize each time the tool, such as a knife and pliers, is used. The dissection room, the vehicles for inspection, and the roads passing by should be disinfected immediately.
Real Time fluorescence quantitative PCR system is a variation of the standard PCR technique that is commonly used to
quantify DNA or RNA in a sample.
Using sequence-specific primers, the number of copies of a particular DNA or RNA sequence can be determined.
By measuring the amount of amplified product at each stage during the PCR cycle, quantification is possible.
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