Answer: Firstly, root cultivation should be carried out: the roots of the grapevine should be cultivated at a height of 25 to 30 cm to protect the rhizomes. Secondly, the trunk must be wrapped: the trunk of the vine should be made of breathable material to protect against cold. Materials such as straw and corn stalk can be used. Do not use dry materials such as plastic paper for wrapping. Winter irrigation is needed again to increase soil heat capacity: timely watering 3 to 4 days before cooling according to soil moisture can reduce frost damage; sweep snow volume of trees in time after snowfall to prevent buds from freezing. Finally, the tree body and the shelf surface can be sprayed with drugs such as brassin and oligosaccharide to improve the tree body resistance.
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PCR ( Polymerase Chain Reaction)
It provides a way to make more copies of a portion of DNA.
We need to have PCR ingredients that include DNA portion, Buffer solution, Primer+DNA (tap polymerase), and Nucleotides during the preparation step.
In the step of PCR sequence, there are three steps in this section.
Step 1 Denaturation
The addition of heat is needed to separate the two strands of the DNA molecule.
Step 2 Annealing
Temperature, is going to be cool in this step, should allow the primers to bind to the specific segments.
Step 3 DNA Synthesis
To make more copies of the DNA.
Those three steps above keep repeating to make enough copies of the DNA fragments. It is the principle of how PCR machine works.
SARS-Cov-2, One test type that uses a sample from a nose or throat swab in a 'PCR test'. The goal is to make enough copies of the viral cDNA in order for it to be detectable.
Superyears General 2215 RT-qPCRadopts all-in-one with an industrial art design, combining the Analytical Instrument, Large touch screen, and Computer. General 2215 RT-qPCR is equipped with the proven thermal-cooled electric technology, which can recognize the speed rate of temperature control faster and obtain high-quality testing results within 40 minutes. To ensure the sensitivity and stability of the testing a design with traditional beam path with a susceptible CMOS image sensor is used. In addition, General 2215 supports multiple PCR analysis and High-resolution Melt Curve (HRM) analysis, compatible with the mainstream reagent kit in the market. It will be the best assistant and your preferred RT-qPCR.
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