The fertilizer and water management of cucumber in winter greenhouse is very important. If the fertilizer and water management is not proper, it will cause bad phenomena such as rotten root, loquat root, and leaf yellowing. Therefore, I would like to remind the friends of vegetable farmers to be careful about the management of cucumber fertilizer and water.
1. Fertilization
Combine organic and inorganic fertilizers. After effective soil testing, formula fertilization is performed according to the previous conditions of the soil and cucumber needs to meet crop growth needs. The general fertilization rule of crops is organic fertilizer plus compound fertilizer plus micro fertilizer plus active beneficial bacterial fertilizer. The selection of fertilizers is mainly based on multiple compound fertilizers and organic fertilizers, and a combination of bottom fertilizer and flush application is adopted. This can activate the soil layer, thereby increasing the area of ​​root extension, and supplementing various nutrients required by the plant. Some vegetable farmers friends blindly increase the amount of fertilizer when fertilizing, such as excessive base fertilizer will cause burnt seedlings; if the amount of fertilization is too large, it will easily cause rotten root, loquat root and so on. Therefore, it is necessary to grasp the appropriate amount when fertilizing, and after the fertilization, it is necessary to cultivate in time. Bio-fertilizer contains a variety of beneficial bacteria, which can activate the soil, increase ground temperature, and regulate soil nutrition. Therefore, proper application of bio-fertilizer can promote root development and disease resistance of cucumber.
Second, watering time In winter, the greenhouse should choose to water the cucumber in sunny morning, because the water temperature is close to the ground temperature at this time, the ground temperature is easy to increase, and there is still enough time to drain moisture in the afternoon. Therefore, try to avoid watering in the evening and cloudy days, so as not to cause the major occurrence and spread of vegetable diseases.
Third, the amount of watering
If the cucumber in the greenhouse is severely deficient in winter, the amount of watering must not be too large. At this time, the soil is prone to lack of oxygen and the root system will suffocate and rot, and the leaves above the ground will even yellow.
Fourth, water temperature
In winter, it is best to choose groundwater for watering cucumbers. The water temperature cannot be lower than 3 ° C, and the amount of water should be small. On the day of watering, the ground temperature should be restored as soon as possible. At the same time, it is necessary to drain as much as possible. If the humidity is too high, it can easily cause downy mildew and powdery mildew.
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Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
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