The safety of the centrifuge is the first, and the loss of control of the centrifugal force can cause great damage. Therefore, it is necessary to pay attention to whether the centrifuge tube is balanced, whether the rotation speed exceeds the setting, and whether the rotor is corroded or the like.
Instruments: high speed refrigerated centrifuge, rotor and centrifuge tube
Separation technology operation project:
1. Balance the centrifuge tube.
2. Centrifuge operation method.
3. Maintenance methods for centrifuges and rotors.
Separation technology considerations:
1. Usually, the centrifuge will have a registration form, please register it correctly before use (user, rotor, speed, time).
2. The centrifuge tube must be well balanced. Pay attention to the position balance when placing the rotor. Never exceed the maximum speed of the centrifuge or rotor.
3. Be sure to leave the centrifuge after reaching the preset speed; if there is any abnormality, stop immediately.
4. Usually you can hear the sound to know if the centrifugal condition is normal, and you can also pay attention to the vibration of the centrifuge.
5. After using a sample of high salt solution such as ammonium sulfate, be sure to wash the rotor and clean the centrifuge chamber.
6. Ultra-high speed centrifuges are extremely high in speed and more complex, requiring additional specialized training before they can be used.
Separation technology use steps:
Usually register before using the centrifuge, please develop registration habits.
1. After setting the operating temperature (usually 4 °C), first place the rotor into the centrifugal chamber, pay attention to the rotor to clamp the shaft; close the door, the temperature of the centrifugal chamber begins to drop, and the pre-cooling time should be sufficient.
2. Load the sample into a suitable centrifuge tube, balance the weight with both balances, cover the centrifuge tube cap and tighten.
â—† Note that the centrifuge tube is only 70% full. Although it is covered, it may leak out due to too strong centrifugal force.
â—† Most of the centrifuge tubes are covered with a lid. Note that the lid of the tube should be balanced.
â—† Centrifuge tubes are usually placed on crushed ice. When removing the balance, wipe off the ice-crushing liquid.
â—† The centrifuge tube that is placed in the order should be equilibrated with another centrifuge tube filled with clean water.
3. Place the well-balanced centrifuge tube symmetrically into the rotor and cover the cover of the rotor, taking care to check for tightness.
â—† If the cover of the rotor is not tightened, it will fall out during centrifugation, causing great damage!
4. Close the centrifuge door and adjust the desired speed and time on the instrument panel, for example 8000 rpm, 30 min.
â—† Note that the speed used must not exceed the limit of the rotor. The maximum speed is usually written on the rotor. For example, the maximum speed of the TGL-20M is 20,
But if the centrifuge is too old, it must be lowered.
â—† The speed and time settings should not be too high. For example, if you can use 7000 rpm, do not use 8000 rpm.
5. After confirming that all the steps are correct, press the start button to start, and the centrifuge will gradually accelerate. At this time, it should be closely monitored.
â—† Some old-fashioned time buttons, when set below 5min, must be rotated for 10min and then transferred back to 5min.
â—† When pressing the start button, the hold time should not be too short. Please keep pressing for about one second before releasing.
â—† It is the most dangerous when starting to accelerate. If you find that the sound is wrong or a big shock, please press stop immediately.
6. Wait until the centrifuge reaches the desired speed and make sure everything is normal before leaving.
7. When the centrifugation is completed, the door should be opened after the rotor is completely still; please take out the centrifuge tube as soon as possible, first observe whether the centrifuge tube is completely, and the position of the sediment, pour out the supernatant as soon as possible, be careful not to turbid the sediment. Water bath thermostat.
â—† Regardless of the supernatant or precipitation, please use a clean beaker to collect the supernatant to avoid mistakes.
â—† Be careful when dumping the supernatant so as not to pour out the sediment together; if you accidentally mix it together, you must come back again.
â—† If there is a leak in the centrifuge tube, find out the cause and clean the rotor or centrifuge chamber immediately.
8. In the neutral between the two centrifugations, do not need to take out the rotor, but cover the door, do not allow hot air to flow into the centrifuge compartment.
9. After all the use, remove the rotor to clean, rinse with tap water, and put it upside down to dry. The centrifuge door should be opened. After the ice is melted, wipe it and clean it. If any liquid leaks out, wash it with water. Also check back the balance of the balance tube and the tabletop, it is easy to get dirty, and it must be carefully cleaned before leaving.
Classification of centrifuges in the experiment:
According to the speed, it can be divided into low speed centrifuge, high speed centrifuge, ultracentrifuge, etc.
According to the temperature, it can be divided into a refrigerating centrifuge and a normal temperature centrifuge;
According to the capacity, it can be divided into micro centrifuge, large capacity centrifuge and ultra large capacity centrifuge;
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