Teprenone is a derivative of terpene, which has a broad-spectrum anti-ulcer effect and is mainly used as a treatment for gastritis and gastric ulcer. Soluble in various organic solvents, molecular weight 330. A commonly used detection method is gas chromatography. The sample is analyzed by HPLC, and the detection wavelength is 205 nm, which can shorten the analysis time, and is convenient and simple.
As-is analysis:
Analytical method: Column: Kromasil 4.6*250mm C18 5μm
Flow rate: 1ml/min
Column temperature: 30 ° C
Detection wavelength: 205nm
Mobile phase: water: acetonitrile = 15:85
After analysis, the crude product was about 73% pure. Since the sample belongs to the terpenoid compound and is at the end of the ultraviolet absorption, the preparation of the normal phase solvent generally requires a general-purpose detector. Therefore, the preparation method is prepared by using a reverse phase solvent, and is performed at 205 nm and 215 nm.
Preparation:
Equipment: EZ Plus 100D
Mobile phase: A-water; B-acetonitrile Flow rate: 18ml/min
Loading amount: 0.2ml liquid is diluted to 2ml, and 0.2ml of the diluent is applied for loading;
Column and packing: 12 g reversed phase column, 40-75 μm
Detection wavelength: 205nm, 215nm
The intermediate main peak was taken for analysis, and the purity was over 98%.
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