Sorting of circulating tumor cells

Circulating tumor cells ( CTC ): According to the pathway of tumor cell metastasis, free tumor cells circulating in the blood or lymphatic vessels are defined as circulating tumor cells and are considered to be an important factor in cancer metastasis.

Application of CTC :

Rapid assessment of in vitro early diagnosis of chemotherapy drugs, individualized treatment, including clinical screening drugs, drug resistance testing to assess prognosis and survival time, tumor recurrence, monitoring of new drug development

CTC detection :

Given the very low levels of CTC, there are typically only a few dozen in 10 ml of blood, and the number of CTCs in advanced patients increases, while 10 ml of blood contains about 100 million white blood cells and 50 billion red blood cells. Therefore, it is best to first enrich CTC in clinical blood samples, and then perform biological and genetic tests and analysis.
Immunomagnetic bead sorting method is currently the most commonly used CTC enrichment method

CD326 ( EpCAM , HEA , ESA ) :

CD326, also known as EpCAM (epithelial adhesion molecule), HEA (human epithelial antigen) or ESA (epithelial specific antigen), is found in most cancer tissues.
CD326 is expressed in epithelial-derived cells, epithelial-derived tumor cells, circulating tumor cells, and cancer stem cells.
Note: Most, but not all, cancer cells express high levels of EpCAM

Sorting human tumor cells with MACS® technology




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