Determination of malachite green and crystal violet and their metabolite residues in aquatic products and feeds by liquid chromatography - tandem mass spectrometry
Food safety analysis workflow
Thermo Scientific Life Science MS not only has a long tradition of technology, but also continues to innovate. Quadrupole mass analysis of true conjugate hyperboloids that fits the perfect theoretical electric field of a textbook is used on all TSQ® triple quadrupole mass spectrometers. Since launching the world's first triple quadrupole MS/MS (TSQ®) mass spectrometer in 1980, TSQ® triple quadrupole mass spectrometer has been widely used in environmental protection, agriculture, quarantine, food safety, etc. Analytical testing, fully capable of testing routine testing of large-volume samples of crops, livestock and poultry products, dairy products and related processed foods, such as pesticide residues in food, veterinary drug residues, mycotoxins, additives, nutritional supplements and organic pollutants And other projects to help you easily meet the increasingly stringent regulatory requirements.
TSQ® Triple Quadrupole Mass Spectrometer and Thermo Fisher Food Safety Testing Strategy
summary
Malachite green and crystal violet are triphenylmethane dyes, often used as disinfectants in the transport of fresh fish. Experimental studies have shown that after malachite green or crystal violet enters the animal body, it is metabolized to a fat-soluble colorless leucomalachite green (also called recessive malachite green) or colorless crystal violet (eucocrystal violet). , also known as recessive crystal violet). Daily testing involves aquatic and feed samples. In this paper, an instrumental method for rapid detection of malachite green and crystal violet and its metabolite residues was developed with reference to SN/T 1768-2006, NY/T 1756-2012, GB/T 19857-2005, and quantified by internal standard method.
Pretreatment
For related reagent materials, standard configuration and reserves, and sample preparation methods, please refer to the above standards. For related instrument methods, please refer to this document. ( TSQ Triple Quadrupole Mass Spectrometer Concise Application Manual - Food Safety Testing )
instrument
The TSQ Triple Quadrupole LC/MS system is equipped with an ultra-high pressure liquid phase system.
Liquid phase condition
Mass spectrometry condition
SRM condition
Experimental result
Typical chromatogram
in conclusion
Among the above-mentioned national standard detection methods, the detection limits of malachite green and crystal violet in different matrices were 2.0-10.0 μg/kg. The method is fully capable of meeting the requirements of domestic and international regulatory standards for sensitivity and reproducibility of conventional regulatory methods.
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