1. All the items required for this operation should be moved into the safety cabinet before operation, avoiding frequent breakage of the airflow through the air curtain with both arms; and disinfecting the surface with 70% alcohol before removing it to remove the pollution.
2, open the fan 5 ~ 10
Minutes, after the air in the cabinet is purified and the airflow is stabilized, the experiment is performed. Slowly extend your arms into the safety cabinet for at least 1 minute to stabilize the airflow in the cabinet before operating.
3. Items not related to this experiment are not placed in the safety cabinet. The items in the cabinet should be placed in a clean area, semi-contaminated area and contaminated area. The items are easy to use during operation and there is no intersection between the three areas. Items should be placed as far back as possible, but the airway opening should not be blocked to avoid interference with normal airflow.
4. The operation should be carried out from the cleaning area to the contaminated area to avoid cross-contamination. To prevent possible spills, a towel or gauze soaked with disinfectant can be placed on the countertop, but the safety cabinet grille should not be covered.
5. During the operation of the cabinet, it is strictly forbidden to use an open flame such as an Alcohol Lamp to avoid the generated heat generating airflow and to interfere with the airflow in the cabinet; and the open flame may damage the HEPA filter.
6. Minimize the movement of people behind the work and quickly switch the door to prevent the airflow in the safety cabinet from being unstable.
7. During the experimental operation, the glass window should not be opened, and the operator's face should be above the working window. When operating in the cabinet, the action should be gentle and soothing to prevent airflow in the cabinet.
8. The safety cabinet should be tested and maintained regularly to ensure its normal operation. If the safety cabinet is found to be abnormal during work, stop working immediately, take appropriate measures, and notify relevant personnel.
9. After the work is completed, close the glass window, keep the fan running for 10 to 15 minutes, and turn on the UV lamp for 30 minutes.
10. The safety cabinet should be cleaned and disinfected regularly. The contaminants in the cabinet can be wiped with 2% 84 disinfectant after the work is completed and the UV lamp is disinfected. The outer surface of the cabinet should be wiped with 1% 84 disinfectant per day.
11. Items used in the cabinet should be removed after disinfection to prevent pathogenic microorganisms from being carried out and polluting the environment. Precautions for biological safety cabinets:
Slow moving principle: In order to avoid affecting the normal wind path state, the hand should be moved as gently as possible during operation in the cabinet.
Parallel placement of items: In order to avoid cross-contamination between items and items, items placed in the cabinet should be placed horizontally as far as possible to avoid cross-contamination during the return air. At the same time, it is avoided to block the back air return grille from affecting the normal wind path.
Avoid vibration principle: Try to avoid the use of vibration instruments (such as centrifuges, vortex oscillators, etc.) in the cabinet, because the vibration will cause the particulate matter accumulated on the filter membrane to shake off, resulting in a decrease in the cleanliness inside the operating room. Failure of the front operating surface balance can also cause contamination of the operator by the safety cabinet.
Principle of movement in different sample cabinets: When two or more items in the cabinet need to be moved, the principle of moving low-pollution items to highly polluting items must be followed to avoid large-scale areas inside the cabinet during the moving process. Pollution.
Open fire use principle: Try not to use open flames in the cabinet. Because fine particle impurities generated during open flame use will be carried into the filter area, these high temperature impurities will damage the filter. When it is unavoidable that it is necessary to use it, it is advisable to use a Bunsen Burner with a low flame.
Alcohol Lamp
Yancheng Rongtai Labware Co.,Ltd , https://www.rongtailab.com