Laboratory centrifuges are instruments that use a rotating rotor to generate centrifugal force to separate substances of different densities and different particle sizes in suspensions or emulsions, and have certain applications in many industries. How should the user choose the laboratory centrifuge product? Let's take a look at the selection method of the laboratory centrifuge. The speed centrifuge is divided into a low speed centrifuge (<10000rpm/min), a high speed centrifuge (1 0000 rpm/min to 30,000 rpm/min), and an ultrahigh speed centrifuge (>30000 rpm/min) depending on the maximum speed. The centrifuge has a rated maximum speed. The maximum speed refers to the speed under no-load conditions, but the maximum speed varies depending on the type of rotor and the quality of the sample.
Centrifuge is a high-tech product integrating multi-disciplinary and multi-technology. It uses the difference of sedimentation speed in different materials to realize the analysis and separation of samples. It is widely used in biomedicine, agriculture, food hygiene, petrochemical and other fields. After the 1970s, the desktop centrifuge was greatly developed. Due to its simple structure, low cost and small size, the desktop centrifuge quickly became a routine instrument in the laboratory. Centrifuge manufacturers and products are numerous, so should be investigated in multiple ways. Firstly, the model should be determined according to the application needs, such as rotor, speed, capacity, temperature, etc. Generally, high speed should not be used for low speed. It should not be used for general purpose. The universal centrifuge takes into account the characteristics of high speed and low speed, large capacity and micro centrifuge. A variety of rotors, so popular with research laboratories, but the price is high. After the model is determined, the technical data, technical performance, maintainability and safety of the product should be further investigated. Stable and reliable. Safety standards, price, shape and appearance. Here are some suggestions for our purchase of your centrifuge:
- Brushless motor is better than DC motor smoke;
- Microcomputer control is better than discrete component control;
- Controlling and displaying the temperature of the sample is more practical than controlling and displaying the temperature of the chamber;
- The accuracy of the speed and temperature is higher than the low;
- The more perfect the safety measures, the better;
- The more standard certification projects, the better;
- The automation program is better than low;
- More than a few functions;
- The width of the rotor selection range is narrower;
- The acceleration of lifting is faster than slow.
After the model is determined, it is not advisable to buy more, because the life of the rotor is limited by the number of times of centrifugation and time. In addition, the rotor is very expensive, so it is mainly considered to buy the commonly used rotor for three or five years.
The after-sales service of the manufacturer is also the main factor of investigation, and the technical maintenance of the centrifuge is very strong.
Not only the maintenance problem, but also the quality and safety issues. If the centrifuge is overhauled or involved in the inspection of safety components, it should be measured in time, such as unbalanced sensing, speed and temperature accuracy, electrical safety and electromagnetic compatibility. Users should be reminded to pay special attention.
For example, a centrifuge rated at 16,000 rpm/min indicates that the rotor rotates 16,000 times per minute at no load, and after the sample is added, the speed must be less than 16000 rpm/min. Different rotors, the maximum speed is also different (one imported centrifuge can be equipped with multiple rotors, and a few manufacturers of domestic centrifuges have successfully developed such technologies. The general separation effect is not the speed but the centrifugal force, so sometimes The speed does not meet the requirements, as long as the centrifugal force can reach the standard, the experiment can achieve the desired effect. Centrifugal force calculation formula: RCF = 11.2 × R × (r / min / 1000) 2 R represents the centrifugal radius, r / min represents Rotating speed.
temperature
Some samples (such as proteins, cells, etc.) will be destroyed in high temperature environment, which is to choose a refrigerated centrifuge, which has a rated temperature range. The heat generated by the centrifuge at high speed is balanced with the refrigeration system of the centrifuge at a certain temperature (the sample for general freeze centrifugation needs to be kept at 3 ° C ~ 8 ° C), and the specific amount can be related to the rotor, such as a centrifuge The rated temperature range is -10 ° C ~ 60 ° C, the horizontal rotor can be rotated to about 3 ° C when installed, if the angle rotor may only be about 7 ° C.
capacity
How many sample tubes need to be centrifuged each time? 7 How much capacity is required for each sample tube? 7 These factors determine the total capacity of a centrifuge, simply the total volume of the centrifuge = the capacity of each centrifuge tube × the number of centrifuge tubes, total The size and workload size are matched.
Rotor
The rotor of the centrifuge is mainly divided into two types: the horizontal rotor: the running blue is in a horizontal state during operation, and is at right angles to the rotating shaft. The sample concentrates the sediment on the bottom of the centrifuge tube: the angle rotor: the centrifugal container forms a fixed angle with the rotating shaft, and the sample will precipitate. Focus on the bottom of the tube and the side wall near the bottom. If you want the separated sample to be concentrated at the bottom of the centrifuge tube, select the horizontal rotor. If you want the sample to concentrate on the bottom of the centrifuge tube and the side wall near the bottom, select the angle rotor.
There are also special tests or special samples that require special rotors such as large-capacity baskets (used for blood stations), ELISA plate rotors, slide rotors, PCR rotors, test tube rotors, and capillary rotors. The rotor has a fixed size, which is combined with the capacity of the centrifuge, such as the 36 × 5 ml angle rotor, which determines the type of rotor and determines the capacity of the centrifuge, so the choice of the rotor is very important.
Control System
High-end centrifuges use a microcomputer control system that not only ensures safe operation of the centrifuge but also automates tasks. Many centrifuges now have better humanized control systems, such as rotor recognition, safety lock function, fault prompt function, acceleration and deceleration curves, and so on. In addition to the above points, pay attention to some details and necessary accessories. The main component of the centrifuge is the motor. The motor is divided into a carbon brush motor and a carbonless brush motor. The former has been eliminated. Most of the current centrifuges are brushless motors, and some motors also have a brake function. Refrigerated centrifuges also differ in terms of refrigeration, and now environmentally friendly technologies are of course fluorine-free refrigeration.
In addition to this, consider the noise problem and try to choose a quieter centrifuge to maintain a comfortable experimental environment. Care should be taken in the accessories. In some experiments, special centrifuge tubes (centrifugal toxic samples or samples requiring ultra-high-speed centrifugation) should be used. Such centrifuge tubes must be equipped with corresponding sleeves for safety. There are also some special sample containers (irregular vials, blood bags, etc.). These details and accessories must be carefully considered when selecting a centrifuge, otherwise normal operation will not be possible.
The purchase of laboratory centrifuges needs to be selected through various aspects, among which there are certain requirements for the speed, temperature, capacity and rotor of the laboratory centrifuge. So how do you choose a laboratory centrifuge? Today, Xiaobian will introduce the selection method of laboratory centrifuges.
Selection method of laboratory centrifuge The speed centrifuge is divided into low speed centrifuge (<10000rpm/min), high speed centrifuge (1 0000 rpm/min 30,000 rpm/min), ultra high speed centrifuge (>30000) according to the maximum speed. Rpm/min), each centrifuge has a rated maximum speed. The maximum speed refers to the speed under no-load conditions, but the maximum speed varies depending on the type of rotor and the quality of the sample. More centrifuge purchases view:
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