Polysporium is a new type of spore-like strain developed by a large number of experiments on spore-like strains in 11 years by experts such as Dr. Zhao Gang from Beijing Institute of Microbiology and Microbiology. Polyspora and conventional spore strains have inherently different superior properties.
Polyporus, live bacteria 3-layer membrane, multi-layer membrane multilayer protection
Polyporus-- is a new multi-spore structure of the spores of Dr. Zhao's multi-stage screening to form the original species by mutagenesis, and then through the 6-stage liquid solid linkage fermentation. Its spore structure produces three extra-layer membranes from conventional single cells, and the membrane wall is significantly thickened. Tests have shown that the function of the bacterium is essentially different from the conventional bacterium, which withstands temperatures up to 140 degrees (20 degrees increase)
PH tolerance 3-11 (one value for each acid and alkali), normal preservation for 35 months, normal resuscitation (increased 23 months storage period) and 40% NPK concentration for normal resuscitation (increased compound concentration) 15%). The difference in application effect is several times higher, and its protection, adaptability and stress resistance are stronger, which is more conducive to soil colonization, and has outstanding effects in preventing soil-borne diseases, promoting soil remediation, and promoting root nutrient environment.
Through several sets of laboratory tests, the advantages of the bacterium can be visualized.
Probiotic fertility comparison test, proven that the fertility of the bacterium is stronger
Test name : soil fertility test
Control object : 2 kinds of microbial agents purchased in Weifang market
Test conditions : the same concentration of bacterial liquid, according to the same inoculum, into the sterilized soil, placed in the incubator and cultured, the plate counted.
Test results : The number of effective viable bacteria in the soil of the bacterium was much higher than that of the control soil.
Functional interpretation : Polysporium has strong soil fixability, high activity and fertility, and great competitive advantage in survival. After being applied to the soil, it is competitively exclusive, and soon forms a dominant flora, forming a zone of inhibition around the roots, protecting the growth of roots and preventing other pathogens.
The intrusion, which competes with other microorganisms for oxygen and nutrients, is competitively exclusive, and it forms a dominant biological population at the root of the crop. In this way, the spores effectively prevent the invasion of other pathogens, obtain the nutrition of the surrounding bacteria, and the growth of the pathogens is inhibited. The spores act like disease prevention and disease resistance.
Comparative test of inhibition zone, it proves that the ability of spores to inhibit harmful bacteria is stronger
Test name : Polysporosis inhibits harmful bacteria test
Control object : sampling of microbial agents applied by farmers in Liaocheng County
Test method : The sterilized filter paper sheets were respectively stained with the spore bacterium solution and the control bacterium liquid, and placed on agar plates coated with pathogenic bacteria, and placed in an incubator for cultivation to determine the diameter of the inhibition zone.
Test results : the diameter of the inhibition zone of the bacterium was significantly larger than the diameter of the inhibition zone of the control bacteria.
Functional interpretation : the antibacterial and anti-disease effect of the genus Polyporus, 1 spore can be accompanied by growth on the mycelium of the pathogen, decomposing and consuming the pathogenic bacteria, causing the mycelium of the pathogen to break and disintegration, so that the pathogen can not further infect The plant can metabolize and secrete bacteriocin (subtilin, polymyxin, chitin, etc.), lipopeptide compounds, organic acids, etc. during the growth of the bacterium, and these metabolites can effectively inhibit the growth of pathogenic bacteria. Or dissolve the pathogens, so as to kill the bacteria, high resistance to heavy mites. The enzyme secreted by 3 spores has chitinase antibacterial protein which has strong inhibitory effect on various plant pathogenic bacteria. The lipopeptide compound secreted by 4 spores can be used for controlling wheat powdery mildew, rice blast, scab, sheath blight, anthracnose, cucumber downy mildew, tomato bacterial wilt, gray mold, and other plant diseases.
Comparative test of phosphate-dissolving, which proves that the ability of polyspora to dissolve phosphorus is stronger
Test name : Comparative test of phosphorus solubilizing capacity
Control object : Yantai market to purchase microbial agents
Test method : The test bacteria were cultured on a solid plate medium containing poorly soluble phosphate or organic phosphorus, and the inoculum was cultured for several days, and the growth of the strain was observed.
Test results : The growth of the polyporus culture medium is very fast, and the ability to decompose nucleic acid and lecithin is stronger than that of the control bacteria.
Functional interpretation : Polysaccharides produce organic acids (amino acids, oxalic acid, fumarate, etc.) through metabolic activities. These acids directly dissolve the poorly soluble phosphate in the soil and release orthophosphate from the insoluble inorganic apatite. .
Test Name : Potassium Contrast Test
Control object : Qingdao market to purchase microbial agents
Test method : The test bacteria were cultured on a solid plate medium containing potassium, and after inoculating the strain for several days, the growth of the strain was observed.
Test results : The growth of the polyporus culture medium is very fast, and the ability to decompose potassium is stronger than that of the control bacteria.
Functional interpretation : Polyporus can promote the conversion of soil phosphorus and potassium, increase the supply of phosphorus and potassium in the soil, and increase crop yield.
Anti-reverse contrast test, proved that: the resistance of the bacterium is stronger
Test name : anti-retroviral test of polyporus
Test conditions : (1) The same concentration of bacterial liquid, an appropriate amount of bacterial solution was applied to an agar plate of pH = 3, pH = 10, and placed in an incubator for cultivation. (2) The same concentration of the bacterial liquid was placed in a water bath at 80 ° C for 30 minutes, and an appropriate amount of the bacterial liquid was applied to the agar plate and placed in an incubator for cultivation.
Test results : (1) At the same pH, the control bacteria showed no colonies, and the spores still grew more colonies. (2) The control bacteria did not grow colonies, and the spores grew well.
Functional interpretation : Polysporus can use a variety of carbon sources, low nutritional requirements, fast growth. The spores exist in the form of spores, which are resistant to high temperature, acid and alkali resistance, extrusion resistance and temperature resistance, and can meet various processing, transportation and storage requirements.
It can be seen from the comparative test that the spores are essentially different from the strains currently used in the current fertilizer, and the transcendence of the comprehensive performance is:
Comparison project | Polyspora | Conventional strain |
form | Multi-membrane spore structure | Monolayer monospore structure |
Metabolite function | A variety of rich polysaccharide metabolites, more functions | Singler metabolite, single function |
Metabolite production | Large yield, comprehensive yield is more than 2 times that of conventional strains | Small output and unstable output |
Bacterial activity | The regular storage period is 35 months and the soil colonization rate is over 90%. Breeding is fast, and the peak of culture can be reached in 18 hours. The number is tens of billions. | The regular storage period is December, and the soil colonization rate is below 30%. Breeding and transmission are slow, and the peak of culture is reached within 24-30 hours, and the number reaches one billion. |
Stress resistance | Tolerant to PH3-11 pH, withstand temperature -40--140 °C | Tolerant to PH 4-9 pH, withstand temperature -10--121 ° C |
Disease resistance | It can prevent soil bacteria and fungi from a variety of soil-borne diseases, and the inhibition zone is large and obvious. | Uncertainty on the prevention and control of soil-borne diseases, the inhibition zone is not obvious. |
Degradability | Degradable soil in the form of solidified phosphorus and potassium, macromolecular organic components. | It can only degrade a single inorganic or organic component. |
Polysporium strain experiment tracking
Crop tracking test, which proves that the advantages of the bacterium are more prominent in rooting and seedling, disease resistance, quality and yield, and soil improvement.
The results of the use of multiple spores in the field for three consecutive years, the data is as follows:
effect which performed | Polyspora | Bacterial fertilizer | Compound fertilizer | Organic Fertilizer |
root | The dry weight and wet weight increased by an average of 20%, the capillary roots were white and many, and the eyesight was obviously increased and developed. | The dry weight and wet weight increased by 6% on average, and the capillary roots increased, and the eye was not obvious. | The root weight is reduced by 1.5% and the roots are weak. | Root weight increased by 5%, root white, hair root is not obvious. |
Seedling stage | The seedlings are well-balanced and the leaves and stems are strong. The height and thickness are increased by more than 10%, 12% thick and 8% thick. Incidence rate <1.5% | 70% neat, the growth is more robust. It is 8% high, 5% thick and 0% thick. Incidence rate <10% | The seedlings are uneven and the growth is uneven. It is 8% high, -2% thick, and 0% thick. The incidence rate is <16%. | 75% are neat and the growth is more balanced. It is 6% high, 6% thick and 2% thick. The incidence rate is <22%. |
The above analysis, the data comes from the Institute of Biotechnology, Chinese Academy of Sciences, Institute of Microbiology and Microbiology, Dr. Zhongnong University. The experiment compares the market brand bacterial fertilizer, compound fertilizer, organic fertilizer and blank sample. Contrast crops: corn, wheat, apples, cucumbers, peanuts, five crops. | ||||
effect which performed | Polyspora | Bacterial fertilizer | Compound fertilizer | Organic Fertilizer |
Adult stage | The root system is developed, with many capillary roots, strong growth, green leaves and stalks. The incidence rate is <2%. | The root system is better, the root is white, and the growth is balanced. Leaf color green, diastolic. The incidence rate is <8%. | The root system is underdeveloped, the growth is uneven, and the leaves are green. The incidence rate is <25%. | More hairy roots, more balanced growth, thick leaves, green color. The incidence rate is <20%. |
Harvest period | The yield increased by 11-36% and the grade was 20-42% higher. The corn kernels were full and not bald. Wheat 1000-grain weight increased by 6%. The color of the fruit is é“, the fruit is positive, the sugar is 80-82% higher. | The production increased by 3-18%, and the grade index increased by 5%-22%. The corn is less bald. The 1000-grain weight of wheat is not obvious. The cucumbers are straight and straight. | The yield increase of 4-15% is reduced by 8-3%. The earing of corn is 35%. The 1000-grain weight of wheat is not obvious. More than 80 fruits, 55%. | The yield is increased by 3%-10%, the grade is 6%-19%. The corn bald ear is 25%, and the wheat 1000 grain is increased by 3%. There are many peanuts and the grains are full. |
soil | Three years of change; acid soil improved 0.6PH, alkaline improved 1.1PH, soil looseness 700px, no knot, water retention increased by 32%. | There was no significant change in one year; acid soil improved 0.3PH, alkali improved 0.5PH, soil looseness 525px, no obvious knot, water retention increased by 22%. | Three years; the acidity increased by 0.2PH, the bulk of the soil was 750px, and the water retention decreased by 3.5%. | Three years: acidity improved by 0.3PH, alkaline improved by 0.2PH, soil looseness of 450px, small knots, and water retention increased by 17%. |
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