In the face of complex samples containing thousands of compounds, the most common analytical methods are liquid chromatography and LC/MS. You may say that these two methods of analysis are very mature, then let's see how we play a new level and reach new heights, haha.
In this issue, let's talk about liquid chromatography. Here, we will not mention the Smart Inject intelligent injection technology that can eliminate the pressure fluctuation of the injection and prolong the life of the column, not to mention the high sensitivity universality that can make the compound look like nothing. CAD electric spray detector...
Let's talk about buying one more than 10 units, the "Transformers" in liquid chromatography - double ternary liquid chromatography .
Why do you buy more than 10 units? Because in addition to meeting the needs of routine testing, dual ternary liquid chromatography can achieve many additional functions, let us take a look: tandem chromatography, parallel chromatography, online solid phase extraction (SPE), on-line desalting, post-column derivatization , post-column compensation, center cutting, two-dimensional liquid phase...
Dual Ternary Liquid Chromatography Function "Fancy Deformation" Diagram
Pre-processing of complex samples, the experienced small partners must have "three lines", the steps are cumbersome, time-consuming, complicated to operate... especially when faced with hundreds of complicated samples to be processed...
With the online SPE technology, we can flexibly configure the suitable switching system (positive punching, recoil, column concentration, etc.) to achieve on-line enrichment and purification of samples, fully automate the pre-treatment and reduce the total analysis time. Moreover, just like putting the elephant in the refrigerator, we only need three steps to achieve it, and all three steps are automatically completed by the instrument, so the mother no longer has to worry about my sample preparation...
Diagram of online SPE working steps in dual ternary liquid chromatography
For complex samples, we want to separate the components as much as possible in the chromatographic separation. In traditional one-dimensional liquid phase separation, many components are concentrated in one peak, which will lose a lot of information in the sample. With full 2D-LC, we are able to truly “strip the complex samples†and further explore the information in complex samples. Think of the "chaotic beginning" of complex samples into the world of "peaks and undulations". Let us thank the "black technology" mysterious power of double ternary liquid chromatography...
Full 2D liquid chromatogram
Full two-dimensional liquid chromatogram of Acanthopanax senticosus water extract
There are too many "black technologies", and Xiaobian can only talk about it here for the time being. In addition to the "fancy" separation, Xiaobian will sacrifice the "carrier-level artifact" in the next issue and continue the "fancy" commentary. Want to know more about the dual ternary mysterious technology, click to read the original text, move to our national university scientific research tour, feel the scene.
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