Han Chunyu, an associate professor at Hebei University of Science and Technology, has shocked NgAgo-gDNA, a new genetic editing technology in the global academic community, and is increasingly questioning. Since his paper was published on the online edition of Nature-Biotechnology on May 2, until now, no laboratory in the world has announced that it can completely repeat Han Chunyu's experiment. Now many scientists have asked Nature-Biotechnology to intervene in the investigation and disclose all the raw data and experimental conditions in the Han Chunyu experiment.
On July 29th, Beijing time, the geneticist Gaetan Burgio of the Australian National University, who once supported Han Chunyu, made a counterattack. He posted a long article "My NgAgo Experience" on Twitter, denying the conclusions he made when he repeated some experiments before July 15, saying that there is no strict evidence that Han Chunyu's NgAgo-gDNA technology has gene editing. Signs and asked Han Chunyu to disclose all raw data and experimental conditions.
Han Chunyu's NgAgo-gDNA technology challenges the most popular gene editing technology, CRISPR-CAS9. When the paper was published, it caused a sensation. Gene editing technology refers to enabling humans to "edit" a target gene to achieve knockout and addition of specific DNA fragments. Currently, CRISPR-CAS9 is the most common gene editing technology and is called "genetic magic scissors." On May 2nd, Han Chunyu's paper "DNA-guided genome editing using the Natronobacterium gregoryi Argonaute" was published online in Nature Biotechnology. By circumventing the fashionable CRISPR-CAS9 technology, the research team used the Argonaute endonuclease of Natronobacterium gregoryi to perform genome editing, referred to as NgAgo-gDNA.
Questioning Han Chunyu's Gaetan Burgio, in his long article published on July 29, introduced the specific process operations and conclusions of the repeated experiments, with seven experimental results.
The blastocyst-based PCR results after pronuclear injection of NgAgo into the fertilized egg.
T7 endonuclease detection of NgAgo-injected fertilized egg PCR products.
Blastocyst-based PCR and electrophoresis gels were injected after injection of mouse fertilized eggs with high concentrations of 5' phosphorylated ssDNA (25 ng/μl) and NgAgo pronuclei.
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